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fresh lb medium with vancomycin  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd fresh lb medium with vancomycin
    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of <t>vancomycin</t> in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.
    Fresh Lb Medium With Vancomycin, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+lb+medium+with+vancomycin/LB+medium/pmc13010146-103-11-16
    Average 95 stars, based on 212 article reviews
    fresh lb medium with vancomycin - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "A WYL transcriptional regulator activates the DNA damage response pathway in Acinetobacter species"

    Article Title: A WYL transcriptional regulator activates the DNA damage response pathway in Acinetobacter species

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkag250

    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of vancomycin in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.
    Figure Legend Snippet: A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of vancomycin in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.

    Techniques Used: Microscopy, Western Blot, Control, Expressing, Concentration Assay, Selection

    Related Articles

    Software:

    Article Title: A WYL transcriptional regulator activates the DNA damage response pathway in Acinetobacter species
    Article Snippet: .. Then, 1 μl of overnight cultures were subcultured into 200 μl fresh LB medium with vancomycin (MP Biomedicals) (0 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 250 μg/ml) grown in a 96-well plate in a Synergy H1 multimode plate reader (BioTek) utilizing Gen 6 software at 30°C shaking for 16 h. OD 600 was measured every 10 min. ..



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    fresh lb medium with vancomycin  (Valiant Co Ltd)
    95
    Valiant Co Ltd fresh lb medium with vancomycin
    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of <t>vancomycin</t> in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.
    Fresh Lb Medium With Vancomycin, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+lb+medium+with+vancomycin/LB+medium/pmc13010146-103-11-16
    Average 95 stars, based on 1 article reviews
    fresh lb medium with vancomycin - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of vancomycin in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.

    Journal: Nucleic Acids Research

    Article Title: A WYL transcriptional regulator activates the DNA damage response pathway in Acinetobacter species

    doi: 10.1093/nar/gkag250

    Figure Lengend Snippet: A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of vancomycin in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.

    Article Snippet: Then, 1 μl of overnight cultures were subcultured into 200 μl fresh LB medium with vancomycin (MP Biomedicals) (0 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 250 μg/ml) grown in a 96-well plate in a Synergy H1 multimode plate reader (BioTek) utilizing Gen 6 software at 30°C shaking for 16 h. OD 600 was measured every 10 min.

    Techniques: Microscopy, Western Blot, Control, Expressing, Concentration Assay, Selection